Swine and poultryviruses, such as porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and highly pathogenic avian influenza virus (HPAIV), are economically important pathogens that can spread via aerosols. The reliability of methods for quantifying particle-associated viruses as well as the sizedistribution of aerosolized particles bearing these viruses under field conditions are not well documented. We compared the performance of 2 size-differentiating airsamplers in disease outbreaks that occurred in swine and poultry facilities. Both airsamplers allowed quantification of particles by size, and measured concentrations of PRRSV, PEDV, and HPAIV stratified by particle size both within and outside swine and poultry facilities. All 3 viruses were detectable in association with aerosolized particles. Proportions of positive sampling events were 69% for PEDV, 61% for HPAIV, and 8% for PRRSV. The highest virus concentrations were found with PEDV, followed by HPAIV and PRRSV. Both air collectors performed equally for the detection of total virus concentration. For all 3 viruses, higher numbers of RNA copies were associated with larger particles; however, a bimodal distribution of particles was observed in the case of PEDV and HPAIV.
The Swine Health Monitoring Project (SHMP) is a National program coordinated by Dr. Bob Morrison from the University of Minnesota, College of Veterinary Medicine. The goal of this initiative is to monitor the incidence and prevalence of relevant swine diseases in the US such as Porcine reproductive and respiratory syndrome (PRRS) or Porcine Epidemic Diarrhea (PED) for example. Participants are voluntarily sharing the health status of their farms in order to better understand, and in the future, control these illnesses. Each week participants receive a report including a one-page summary of a scientific fact of interest. From now on, the Science Page will be published on the blog here, every Friday.
The Allen D. Leman swine conference is only six months away! Our team is preparing an exciting conference program for you this year and we will update you regularly on the speakers and topics that will be covered during this great event bringing science-driven solutions to the complex challenges facing the swine industry. We already shared with you that the recipient of this year’s Science in Practice award is Dr. Bob Thompson.
We are glad to announce that our first key note speaker for the conference will be Gary Louis, Executive Vice President of Seaboard Foods LLC where he is in charge of the live operations. We are delighted that he has accepted our invitation to share his tremendous experience and his vision of the swine production.
More information about the Allen D. Leman swine conference can be found here.
The UMN CVM students did a fantastic job at the 2017 American Association of Swine Veterinarians (AASV) meeting this past weekend. Four students presented their projects as an oral presentation. Zhen Yang, Alyssa Anderson, Hunter Baldry and Chris Deegan were all recognized by a jury for their hard work and commitment to the swine industry.
Taylor Homann, Donna Drebes, and Kevin Gustafson all got the opportunity to present their work as poster presentations.
Lastly, two out of the three awards given by Boehringer Ingelheim to advance the research on swine respiratory pathogens were given to Dr. Marie Culhane and Dr. Carlos Vilalta for their project on swine influenza and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) respectively.
What disinfectant to use against Senecavirus A? Does it vary based on surface type or temperature? These are the questions that a group of researchers from the University of Minnesota – Veterinary Diagnostic Laboratory are answering in the latest issue of the Journal of Swine Health and Production (JSHAP).
The project tested: 3 disinfectants: Household bleach, a phenolic disinfectant, and a quaternary ammoniun-aldehyde 5 surfaces: aluminium, stainless steel, rubber, cement, and plastic 2 temperatures: 25°C (77F) and 4°C (39F)
Objectives: To evaluate the virucidal efficacy of three commercial disinfectants against Senecavirus A (SVA) on five different surfaces at ~25°C and 4°C.
Materials and methods: Household bleach, a phenolic disinfectant, and a quaternary ammonium-aldehyde disinfectant were tested at manufacturer’s recommended concentrations against a contemporary strain of SVA on aluminum, stainless steel, rubber, cement, and plastic surfaces at ~25°C and 4°C. Virus propagation and titration were performed on swine testicular cells. Viral titers were calculated before and after exposure to the disinfectant being tested.
Results: At ~25°C, household bleach at 1:20 dilution inactivated ≥ 99.99% of the virus within 10 to 15 minutes on aluminum, rubber, and plastic. On stainless steel and cured cement, it inactivated 99.97% and 99.98% of the virus, respectively. At 4°C, bleach inactivated ≥ 99.99% of the virus within 5 to15 minutes on all surfaces except rubber; on rubber, inactivation was 99.91% after 15 minutes. The phenolic disinfectant at the manufacturer’s recommended concentration inactivated only ≤ 82.41% of the virus at either temperature and on any surface, even after a 60-minute contact time. Results for the quaternary ammonium disinfectant were intermediate: 78.12% to 99.81% of the virus was inactivated within 60 minutes at both temperatures and on all surfaces. To detect differences between disinfectants, paired Wilcoxon tests were performed. At 10- and 15-minute time points, efficacies of the three disinfectants differed significantly.
Implications: Significant variation exists in the antiviral efficacies of different disinfectants. Hence, they should be tested against various pathogens before use in the field.