Summary:
- From Dr. Torremorell’s lab at the University of Minnesota, College of Veterinary Medicine
- Porcine Reproductive and Respiratory Syndrome virus (PRRSV), Porcine Epidemic Diarrhea virus (PEDV), and High-Path Avian Influenza virus (HPAIV) are transmitted via aerosols.
- Air samples taken out of positive farms were successful in isolating HPAIV and PEDV 61% and 69% of the time respectively whereas PRRSV was found 8% of the time.
- For all three viruses, higher quantity of genetic material was found when the size of the particle was larger.
More articles on air samples as a method to study respiratory pathogens
Abstract:
Swine and poultry viruses, such as porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and highly pathogenic avian influenza virus (HPAIV), are economically important pathogens that can spread via aerosols. The reliability of methods for quantifying particle-associated viruses as well as the size distribution of aerosolized particles bearing these viruses under field conditions are not well documented. We compared the performance of 2 size-differentiating air samplers in disease outbreaks that occurred in swine and poultry facilities. Both air samplers allowed quantification of particles by size, and measured concentrations of PRRSV, PEDV, and HPAIV stratified by particle size both within and outside swine and poultry facilities. All 3 viruses were detectable in association with aerosolized particles. Proportions of positive sampling events were 69% for PEDV, 61% for HPAIV, and 8% for PRRSV. The highest virus concentrations were found with PEDV, followed by HPAIV and PRRSV. Both air collectors performed equally for the detection of total virus concentration. For all 3 viruses, higher numbers of RNA copies were associated with larger particles; however, a bimodal distribution of particles was observed in the case of PEDV and HPAIV.