PCR clamping for selectively sequencing wild-type PRRSV in vaccinated herds

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, we are sharing a report by Harmon et al. from Iowa State University regarding PCR clamping. This project was funded by AAVLD Thermo Fisher Innovation Grant in Veterinary Diagnostic Medicine and ISU-VDL.

Key points

  • Conventional ORF5 sequencing may not differentiate between wild-type or vaccine-like.
  • Blocking the amplification of vaccine-like sequences it is possible to increase the likelihood of wild-type amplification.
  • Clamping allows the amplification of the wild-type with mixtures containing as little as 10% of a mixture with the vaccine-like.
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Effect of litter aggregation and pooling on detection of PRRSv in piglet processing fluids

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

The Science Page this week was written by the MSHMP team and covers pooling of processing fluids. A more detailed report can be found on the blog. Currently, a study to evaluate the number of negative processing fluids to declare a farm stable is ongoing. If you are interested in participating in this study, contact Dr. Juan Sanhueza (jsanhuez-at-umn-dot-edu).

Continue reading “Effect of litter aggregation and pooling on detection of PRRSv in piglet processing fluids”

MSHMP Incidence Year 2018/2019 Annual Summary

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Today, we are sharing the 2018/2019 summary for the Morrison Swine Health Monitoring Program from the MSHMP team lead, Dr. Cesar Corzo and what his vision for the future is.

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Best of Leman 2018 series #9: K. VanderWaal – Can we predict PRRS and PED outbreaks?

We launched a new series on the blog last year. Once a month, we are sharing with you a presentation given at the Allen D. Leman swine conference, on topics that the swine group found interesting, innovative or that lead to great discussions.

We can find all of the presentations selected from last year’s conference on the blog here.

Our ninth presentation is from Dr. Kim VanderWaal, our colleague at the University of Minnesota, who gives us a glimpse into a future when producers might be able to know when their farms are at risk of disease outbreaks.

Continue reading “Best of Leman 2018 series #9: K. VanderWaal – Can we predict PRRS and PED outbreaks?”

PRRS incidence rate by status at break

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Key points

  • Current PRRS Chart 3 incidence risk by status does not take into account the time at risk farms contribute to each status.
  • Incidence rate takes into account the time a farm is at risk of disease.
  • Chart 3 will be replaced by a incidence rate chart that reflects more accurately the PRRS outbreak rate by status.
Continue reading “PRRS incidence rate by status at break”