oral fluids – Swine in Minnesota

Should we be concerned about the effect of sample handling on PRRSV detection by RT-qPCR?

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

In this week’s Science, Iowa State researchers Page Berenice Munguía-Ramírez, Betsy Armenta-Leyva, Alexandra Henao-Díaz, Ting-Yu Cheng, Jianqiang Zhang, Gaurav Rawal, Fangshu Ye, Luis Giménez-Lirola and Jeffrey J. Zimmerman summarize three studies looking at the effect of temperature and time on the detection of PRRSV by PCR in serum, oral fluids and fecal samples.

Evaluation of oral fluid as an aggregate sample for early detection of ASF

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Since ASF has been found in the Dominic Republic last week, swine producers are concerned about it spreading into the United States. Early detection would be essential to ensure a rapid response and containment of the disease. This week, the MSHMP team shares the summary of a Canadian study looking at using oral fluids as a tool to diagnose ASF.

Key Points

Oral fluid samples may be used as a low labor, cost effective alternative sample source for rapid detection of ASFV during ASF surveillance.
ASFv genome was detected in oral fluids at low-to-moderate levels as early as 3-5 days post infection, before clinical symptoms started.
Further research is needed to understand limitations and best practices of oral fluid sampling for ASFv

Relationships among Microbial Communities in Pigs

Today, we are sharing a recent article published in the journal Microorganisms by Dr. Valeris-Chacin and the MycoLab. The publication, available in open access, focuses on the relationships among fecal, air, oral, and tracheal microbial communities in pigs.

Natural transmission and detection of Mycoplasma hyopneumoniae in a naïve gilt population

Dr. Alyssa Betlach, member of the Maria Pieters Myco Lab, recently published a study on the natural transmission of Mycoplasma hyopneumoniae in Veterinary Microbiology. The study evaluated transmission rate in a naïve gilt population and compared various sample types to detect infection.

Molecular detection of Porcine Reproductive and Respiratory Syndrome virus, Porcine Circovirus 2 and Hepatitis E virus in oral fluid compared to their detection in feces and serum

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Doctors Plut, Kamnikar-Ciglenecki and Stukeli from the University of Ljubljana (Slovenia) highlight the usefulness of pig oral fluid (OF) to detect Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Porcine Circovirus Type 2 (PCV2) and Hepatitis E virus (HEV) in different pig age categories. Read here the full article published in BMC Veterinary Research.

Key Points

OF, feces and serum were evaluated for the detection of PRRSV, PCV2 and HEV in six farms.
OF samples had good agreement with serum sample PCR results for the detection of all three viruses.
The study highlights that pooled samples can potentially be used to investigate viral presence on farms.