A recently published study conducted by Mark Schwartz and collaborators evaluated the feasibility of using air sampled from right outside the exhaust fans on finishing barns to detect PRRSV. The full article is available in open access on the journal’s website.
Continue reading “Detection of PRRSV-2 in air emissions from grow-finish barns”Tag: oral fluids
Swine Practitioner Practices on Oral Fluid Sampling in U.S. Swine Farms: A Nationwide Survey
Today, we are sharing a recent publication from the MSHMP team in the journal Pathogens. This project set out to gather information on how swine veterinarians use oral fluids in the USA through a nationwide survey.
Continue reading “Swine Practitioner Practices on Oral Fluid Sampling in U.S. Swine Farms: A Nationwide Survey”Assessment of real-time PCR test in oral fluid samples for screening the serotypes of Actinobacillus pleuropneumoniae circulating in swine herds
This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.
Researchers José Luis Arnal, Ana Belén Fernández, Sonia Lacotoure, Alfredo Ángel Benito, Sofía Lázaro, and Marcelo Gottschalk share their study evaluating oral fluids combined with qPCR for detecting and serotyping A. pleuropneumoniae in swine herds.
Continue reading “Assessment of real-time PCR test in oral fluid samples for screening the serotypes of Actinobacillus pleuropneumoniae circulating in swine herds”Should we be concerned about the effect of sample handling on PRRSV detection by RT-qPCR?
This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.
In this week’s Science, Iowa State researchers Page Berenice Munguía-Ramírez, Betsy Armenta-Leyva, Alexandra Henao-Díaz, Ting-Yu Cheng, Jianqiang Zhang, Gaurav Rawal, Fangshu Ye, Luis Giménez-Lirola and Jeffrey J. Zimmerman summarize three studies looking at the effect of temperature and time on the detection of PRRSV by PCR in serum, oral fluids and fecal samples.
Continue reading “Should we be concerned about the effect of sample handling on PRRSV detection by RT-qPCR?”Evaluation of oral fluid as an aggregate sample for early detection of ASF
This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.
Since ASF has been found in the Dominic Republic last week, swine producers are concerned about it spreading into the United States. Early detection would be essential to ensure a rapid response and containment of the disease. This week, the MSHMP team shares the summary of a Canadian study looking at using oral fluids as a tool to diagnose ASF.
Key Points
- Oral fluid samples may be used as a low labor, cost effective alternative sample source for rapid detection of ASFV during ASF surveillance.
- ASFv genome was detected in oral fluids at low-to-moderate levels as early as 3-5 days post infection, before clinical symptoms started.
- Further research is needed to understand limitations and best practices of oral fluid sampling for ASFv