Evaluation of oral fluid as an aggregate sample for early detection of ASF

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Since ASF has been found in the Dominic Republic last week, swine producers are concerned about it spreading into the United States. Early detection would be essential to ensure a rapid response and containment of the disease. This week, the MSHMP team shares the summary of a Canadian study looking at using oral fluids as a tool to diagnose ASF.

Key Points

  • Oral fluid samples may be used as a low labor, cost effective alternative sample source for rapid detection of ASFV during ASF surveillance.
  • ASFv genome was detected in oral fluids at low-to-moderate levels as early as 3-5 days post infection, before clinical symptoms started. 
  • Further research is needed to understand limitations and best practices of oral fluid sampling for ASFv
Continue reading “Evaluation of oral fluid as an aggregate sample for early detection of ASF”

A quick guide to Mycoplasma hyopneumoniae diagnostics

Sample types for early detection of Mycoplasma hyopneumoniae is a popular post on this blog. Dr. Pieters, head of the MycoLAb at the University of Minnesota created an online quick guide to help swine practitioners decide which sample type they should collect if they are looking for Mycoplasma hyopneumoniae.

This guide is available at http://z.umn.edu/MycoplasmaDiagnostics

Continue reading “A quick guide to Mycoplasma hyopneumoniae diagnostics”

Mycoplasma hyorhinis and Mycoplasma hyosynoviae dual detection patterns in dams and piglets

Today, we are sharing an original research article published by the MycoLab and Dr. Maria Pieters in PLOS One regarding detection patterns for 2 species of mycoplasmas in sows and piglets.

The objectives of this study were to:

  • describe when Mycoplasma hyorhinis and Mycoplasma hyosynoviae can be detected in piglets and is sows,
  • assess if there was a correlation between detection of the mycoplasmas in the sow and in the piglet, and
  • assess if there was a correlation between lameness and mycoplasma detection.

Conclusions

Under the conditions of this investigation, dams appeared to be consistently positive for both M. hyorhinis and M. hyosynoviae prior to weaning.

In contrast, higher detection was observed in piglets at week 3, in comparison to week 1 post-farrowing, with M. hyorhinis, while detection of M. hyosynoviae was remarkably minimal.

The relative risk of developing lameness in postweaning piglets was highly associated with the detection of M. hyorhinis at 3 weeks of age

This research article is available in open-access on the PlOS One website.

Continue reading “Mycoplasma hyorhinis and Mycoplasma hyosynoviae dual detection patterns in dams and piglets”

Science Page: Detecting influenza virus with a portable device

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

We are presenting today the work done in Dr. Cheeran’s lab on the detection of influenza virus in farms. The objective of their research project is to develop a portable diagnostic platform that is capable of performing on-site testing of influenza viruses in swine with minimum sample handling and laboratory skill requirements.

The device is usingĀ giant magnetoresistance (GMR) technology. In a nutshell, if influenza viruses are present in the sample, they will bind to sensors, cause a disruption in resistance, and create an electric signal in the device that will be able to wirelessly transmit the result to a smartphone or computer.

Key points from this week edition:

  • Portable, hand held device for detection of influenza A virus (IAV) based on giant magnetoresistance (GMR) biosensor has been developed.
  • Although in its developmental stage, if successful this test has the potential for rapid on-site testing of influenza viruses in swine.

The first sensitivity tests of the device look very promising!