Frequency and origin of live imports of breeding-stock pigs to the United States

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, we are sharing a report from the MSHMP team in collaboration with the Swine Health Information Center regarding the origin of the pigs that are imported for breeding purposes.

Key Points

  • International live pig transport is a possible route for disease entry into the U.S.
  • The first step in assessing this risk is to categorize and analyze breeding stock import data
  • Small numbers of imported breeding pigs have come from a variety of countries, but the majority come from Canada
Continue reading “Frequency and origin of live imports of breeding-stock pigs to the United States”

PCR clamping for selectively sequencing wild-type PRRSV in vaccinated herds

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, we are sharing a report by Harmon et al. from Iowa State University regarding PCR clamping. This project was funded by AAVLD Thermo Fisher Innovation Grant in Veterinary Diagnostic Medicine and ISU-VDL.

Key points

  • Conventional ORF5 sequencing may not differentiate between wild-type or vaccine-like.
  • Blocking the amplification of vaccine-like sequences it is possible to increase the likelihood of wild-type amplification.
  • Clamping allows the amplification of the wild-type with mixtures containing as little as 10% of a mixture with the vaccine-like.
Continue reading “PCR clamping for selectively sequencing wild-type PRRSV in vaccinated herds”

Best of Leman 2018 series #10: A. Gomez – Swine gut microbiome studies, where are we at?

We launched a new series on the blog last year. Once a month, we are sharing with you a presentation given at the Allen D. Leman swine conference, on topics that the swine group found interesting, innovative or that lead to great discussions.

We can find all of the presentations selected from last year’s conference on the blog here.

Our tenth presentation is from Dr. Andres Gomez, from the College of Food, Agricultural and Natural resource Sciences (CFANS) regarding the latest development in the swine gut microbiome studies.

Continue reading “Best of Leman 2018 series #10: A. Gomez – Swine gut microbiome studies, where are we at?”

How does giving tulathromycin at birth impact the fecal microbiota?

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week’s Science Page presents the second part of a project done by Zeineldin et al. from Dr. Jim Lowe’s program at the University of Illinois. The first part is available here.

Keypoints

  • Fluctuations in antimicrobial resistance genes (ARGs) associated with the developing gastrointestinal microbiota have not been elucidated
  • The swine fecal microbiota and their accompanying ARGs were diverse and established soon after birth
  • Results indicate that the perinatal TUL metaphylaxis has no measurable beneficial or detriment impacts on fecal microbiota structure and abundance of ARGs in pre-weaned piglets
Continue reading “How does giving tulathromycin at birth impact the fecal microbiota?”

Best practices to submit samples: new UMN VDL handouts available

If you are unsure about how to submit processing fluids or laryngeal swabs, the University of Minnesota Veterinary Diagnostic Laboratory put together one-age illustrated handouts to guide you towards best practices for each commonly used sample type. Each one of them is available in a downloadable pdf format for convenience.

The handouts can be found on the VDL website.