PCR – Swine in Minnesota

Assessment of real-time PCR test in oral fluid samples for screening the serotypes of Actinobacillus pleuropneumoniae circulating in swine herds

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Researchers José Luis Arnal, Ana Belén Fernández, Sonia Lacotoure, Alfredo Ángel Benito, Sofía Lázaro, and Marcelo Gottschalk share their study evaluating oral fluids combined with qPCR for detecting and serotyping A. pleuropneumoniae in swine herds.

Summary: Comparison of a novel rapid sampling method to serum and tonsil scraping to detect PRRSV in acutely infected sows

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

In this week’s Science Page Peng Li, Ana Paula Poeta Silva, Daniel Moraes, Paul Yeske, Henry Osemeke, Edison Magalhaes, Gustavo Silva, and Daniel Linhares share a summary of their study comparing different PRRSV sampling methods.

Featured abstracts of 27th International Pig Veterinary Society Congress – Leipzig Germany, June 4-7, 2024 – Part 2

The International Pig Veterinary Society (IPVS) is an association of pig health and production specialists founded in 1967. Since 1972, congresses have been held every other year. Recently, the 27^th IPVS Congress was held in Leipzig, Germany. The 28^th Congress is scheduled for Ho Chi Minh City, Vietnam in 2026, and the 29^th Congress is scheduled for Minneapolis, United States in 2028. The objectives of the IPVS are 1) the holding of international congresses for the exchange of knowledge related to pig health and production; and 2) the promotion of the formation of Pig Veterinary Societies in all pig-producing countries and the promotion of cooperation between such societies. We have selected some examples of the work presented at the recent IPVS and share them with you on this and last week’s science page.

Is the PRRSV isolate obtained in cell culture the same as the virus present in the original clinical sample?

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

In today’s Science Page the Veterinary Diagnostic Laboratory and the Production Animal Medicine team at Iowa State University look into whether cell culture isolates and viruses in the associated clinical samples are sequence equivalent or not.

PCR detection of Mycoplasma hyopneumoniae in piglet processing fluids in the event of a clinical respiratory disease outbreak

Dr. Vilalta and collaborators from the University of Minnesota provide new insights for the potential use that processing fluid (PF) may have to detect M. hyopneumoniae in breeding farms by bringing us a clinical case published in VetRecord Case Reports.

Highlights

Genetic material of Mycoplasma hyopneumoniae from PF was quantified by real-time PCR.
The origin of M. hyopneumoniae genetic material detected in PF needs to be clarified.
Processing fluids are a potential sample to detect M. hyopneumoniae in breeding herds.