Litter level agreement of PRRS PCR results in testicles, tails and udder wipes samples

This recent publication by former MSHMP post-doc Dr. Carles Vilalta compares various sample types used to assess PRRS status of piglets at the litter level. The paper is available in the Preventive Veterinary Medicine journal.

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Senecavirus A in processing fluids during an outbreak of SVA – a call for study participation!

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Doctors Guilherme Preis and Cesar Corzo from the University of Minnesota share with us preliminary data from a Senecavirus A (SVA) outbreak investigation in a sow herd. This research emphasizes the importance of understanding the within-herd epidemiology of this virus.

Highlights

  • SVA is still present in the U.S. swine herds at lower levels.
  • SVA RNA has been consistently detected in processing fluids in the event of a disease outbreak in a sow herd.
  • Viral dynamics and shedding cessation in the breeding herds need to be better characterized.
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PCR detection of Mycoplasma hyopneumoniae in piglet processing fluids in the event of a clinical respiratory disease outbreak

Dr. Vilalta and collaborators from the University of Minnesota provide new insights for the potential use that processing fluid (PF) may have to detect M. hyopneumoniae in breeding farms by bringing us a clinical case published in VetRecord Case Reports.

Highlights

  • Genetic material of Mycoplasma hyopneumoniae from PF was quantified by real-time PCR.
  • The origin of M. hyopneumoniae genetic material detected in PF needs to be clarified.
  • Processing fluids are a potential sample to detect M. hyopneumoniae in breeding herds.
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Effect of litter aggregation and pooling on detection of PRRSv in piglet processing fluids

The Science Page this week was written by the MSHMP team and covers pooling of processing fluids. A more detailed report can be found on the blog. Currently, a study to evaluate the number of negative processing fluids to declare a farm stable is ongoing. If you are interested in participating in this study, contact Dr. Juan Sanhueza (jsanhuez-at-umn-dot-edu).

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Effect of litter aggregation and pooling on detection of PRRS virus in piglet processing fluids

The MSHMP team, led by Dr. Carles Vilalta published a new article in the Journal of Veterinary Diagnostic Investigation regarding the effect of pooling on the detection of PRRS in processing fluids.

The MSHMP team has been one of the first to publish data on the detection of PRRS in processing fluids. You may find all of our previous articles on processing fluids here.

Objectives of the study

  • Identify the limit of PRRS virus detection in pooled processing fluids
  • Evaluate the effect of pooling on the initial Ct value
Continue reading “Effect of litter aggregation and pooling on detection of PRRS virus in piglet processing fluids”