Infection dynamics and incidence of wild-type PRRS virus in growing pigs

This new publication from the Torremorell’s lab is focusing on growing pig herds in the Midwest and how wild-type PRRS virus can spread among them. The full publication is available on the journal’s website.

Highlights

  • Wild-type PRRSV infections in growing pigs are common and more prevalent towards the middle to end of the growing phase.
  • Site-level surveillance is useful to understand PRRSV infections in vaccinated pigs.
  • Biosecurity practices in growing pig sites are key to prevent lateral infections.
Continue reading “Infection dynamics and incidence of wild-type PRRS virus in growing pigs”

Phylogenetic Structure and Sequential Dominance of Sub-Lineages of PRRSV Type-2 Lineage 1 in the United States

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

Keypoints

  • Lineage 1 PRRSv, the most prevalent PRRSV lineage in the U.S, can be sub-divided into eight sub-lineages
  • We documented the cyclic emergence and turnover of different lineages and sub-lineages (about every 3 years) based on both sequence count data and estimated past viral population sizes inferred from genetic diversity through time.
  • The eight sub-lineages differed in key GP5 amino acid sites that are thought to be involved in the immune response to the virus.
Continue reading “Phylogenetic Structure and Sequential Dominance of Sub-Lineages of PRRSV Type-2 Lineage 1 in the United States”

Understanding whether PRRS viruses in a neighborhood are closely related

This week, the MSHMP team is sharing results from a study looking at how related PRRSv ORF5 sequences from a same area are.

Key Points

  • 28 space-time clustering of PRRSv based on producers’ routine molecular surveillance were identified in the course of 2010-2019.
  • Being inside or outside a space-time cluster significantly explains the genetic variability of most, but not all cases.
  • Assessing space, time, and genetic relatedness relationships in PRRSV transmission is complex, and overall trends might miss important case information.
Continue reading “Understanding whether PRRS viruses in a neighborhood are closely related”

PCR clamping for selectively sequencing wild-type PRRSV in vaccinated herds

This week, we are sharing a report by Harmon et al. from Iowa State University regarding PCR clamping. This project was funded by AAVLD Thermo Fisher Innovation Grant in Veterinary Diagnostic Medicine and ISU-VDL.

Key points

  • Conventional ORF5 sequencing may not differentiate between wild-type or vaccine-like.
  • Blocking the amplification of vaccine-like sequences it is possible to increase the likelihood of wild-type amplification.
  • Clamping allows the amplification of the wild-type with mixtures containing as little as 10% of a mixture with the vaccine-like.
Continue reading “PCR clamping for selectively sequencing wild-type PRRSV in vaccinated herds”

Science Page: Making epidemiological sense out of large datasets of PRRS sequences

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, we are sharing an epidemiological report regarding a large PRRS sequence dataset from Dr. Igor Paploski in the VanderWaal research group.

Key points:

  • Occurrence of PRRS lineages is not equal in different years, systems or production types
  • Occurrence of specific PRRS lineages is associated with movement of animals
  • Continuous surveillance for PRRS occurrence is important in understanding its determinants and might be able to provide insights that can
    help on its prevention

By utilizing a dataset of 1901 PRRS sequences provided by the Morrison Swine Health Monitoring Project (MSHMP) participants over 3 recent years, the spatiotemporal patterns in the occurrence of different lineages of PRRSV was described and the extent to which the network of pig movement between farms determines the occurrence of PRRS from similar lineages was investigated.

PRRS lineages occurred at different frequencies across geographically overlapping production systems. Preliminary analysis showed that the relative frequency in which specific lineages occur increase while others are decrease over time. The rate at which these changes occur appears to be system-specific. Some lineages were also more common in farms of specific production types (i.e. sow farm or nurseries). As expected, farms that were connected via pig movements were more likely to share the same lineages than expected by chance across all years.

These findings suggest that system-specific characteristics partially drive PRRS occurrence over time and across farms of different production types. Our results also
indicate that animal movement between farms is a driver of PRRS occurrence, strengthening this hypothesis of viral transmission.

Additional research is needed to quantify risks and develop mitigation measures related to animal movement.

Large PRRS sequencing dataset