49th AASV annual meeting: Come see us in San Diego!

San Diego skyline from Pt64 On Saturday starts the 49th annual meeting of the American Association of Swine Veterinarians in San Diego, CA. Once again, the swine group from the University of Minnesota, College of Veterinary Medicine will be well represented. This year’s theme is Global Knowledge: Individual Application.

Dr. Marie Culhane, member of this year’s program committee will also be co-chair a seminar on Sunday morning regarding the 4-dimensional revolution in food animal health and production: the synthesis of diagnostics, devices, digital platforms, and data analytics.  Dr. Maria Pieters will co-chair the DVM student seminar hold on Sunday afternoon.

Numerous faculty member, graduate students, researchers and DVM students will be presenting throughout the conference.

Presenter Topic When Session
Montse Torremorell Comparison of aggregate/population samples to detect viruses in suckling pigs Saturday, 2:05pm Monitoring and Surveillance 2.0
Albert Rovira Using Optisample to determine sample for specific needs Saturday, 4:15pm Monitoring and Surveillance 2.0
Montse Torremorell Air filtration testing: understanding filter efficacy and leakage Saturday, 1:25pm Biosecurity
Juan Sanhueza MSHMP Sunday, 10:15am The 4-dimensional revolution in food animal health and production
Jerri Torrison PigSAVI Sunday, 10:40am The 4-dimensional revolution in food animal health and production
Fabio Vannucci Diagnostic testing for challenging cases Sunday, 9:15am Diagnostics
Fabian Chamba Pardo Breeding herd factors associated with influenza in piglets at weaning Sunday, 9:15am Research topics
Nirmala Jayaveeramuthu Is influenza vaccination a key driver of influenza genetic diversity in piglets? Sunday, 9:30am Research topics
Jorge Garrido Mantilla Novel approaches for influenza surveillance in swine breeding herds Sunday, 9:45am Research topics
Matthew Sturos Shedding and persistence of Senecavirus A in boars Sunday, 11:45am Research topics
Zhen Yang Geographic distribution and genetic diversity of PCV3 from clinical samples in US swine farms Sunday, 4:15pm Student seminar
Fernando Leite The impact of zinc amino acid complex supplementation on the porcine response to subclinical Lawsonia intracellularis infection Sunday, 1:15pm Industrial Partners
Erika Vasquez Comparison of IPMA and commercial blocking ELISA for Lawsonia intracellularis Sunday, 3:00pm Industrial Partners
Fabio Vannucci Senecavirus A: pathologist/research perspective Monday, 4:45pm Emerging diseases
Peter Davies Measuring antibiotic use in pork production: why, how, and for whom? Tuesday, 10:30am General session

Do not forget to take a look at the various posters we will be presenting!

Presenter Topic Poster #
Taylor Homann Comparison of individual and pooled oral fluid samples, and Swiffer environmental samples of drinkers for the detection of IAV-S and PRRSV by PCR 12
Kevin Gustafson In vitro culture of porcine B cells 26
Marjorie Schleper Evaluation of farrowing stall sanitation protocols with ATP bioluminescence 37
Talita Resende Co-detection of PCV2 and PCV3 in pig samples 64
Juan Sanhueza Factors that impact PRRS time to stability 65
Frances Shepherd Phenotypic relationship and hypervariability of the VP7 gene in porcine rotavirus B and C 66
Shaoyuan Tan Benchmarking pen-side sequencing for infectious disease diagnosis 67
Carles Vilalta Model to estimate PRRSV introduction in filtered farms with negative pressure 72

Hope to see you there!

Science Page: Prevalence comparison among different MSHMP cohorts

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, we are sharing a report from the MSHMP team regarding the differences in PRRS prevalence among various cohorts.

Key points:

  • Prevalence among cohorts does not differ.
  • Seasonal patterns can be seen in different cohorts located in different regions.

Prevalence PRRS status cohortA comparison from a prevalence standpoint between the cohort of farms belonging to the 13 systems participating at the start of the MSHMP (CS) and the cohort of farms from systems that joined the program later (CL), was performed with the objective of assessing whether the patterns between cohorts compare.

As seen in Figure 1–CS, there was a clear shift towards more use of MLV over LVI for sow herd stability purposes. The proportion of farms using LVI in the CS versus the CL is 5% and 10%, respectively. When assessing the proportion of farms in each AASV PRRS category (Holtkamp et al., 2011) both groups are comparable (Table 1). Also the temporal pattern of infection can be seen in both cohorts as described by Tousignant et al (2014).

In summary, both cohorts of farms (CS versus CL) yield similar results which continue to highlight the robustness of the program and the representativeness of the systems contributing to this program.

Best of Leman 2017 series #5: J. Pittman – We’ve posted a lot of sows.

We launched a new series on the blog in October. Once a month, we are sharing with you a presentation given at the 2017 Allen D. Leman swine conference, on topics that the swine group found interesting, innovative or that lead to great discussions.

The fifth presentation we are sharing is from Dr. Jeremy Pittman from Smithfield’s, NC. In the context of increased sow mortality, Dr. Pittman spent a lot of time investigating the root causes of this change and he shares his experience in this talk.

To listen to this talk, please click on the picture below.

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Science Page: Investigating the role of the environment and the lactating sow in PRRSV infections during an outbreak (Part 2)

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, we are sharing part 2 of the report on the role of the environment and the lactating sow in PRRSV outbreak. You may find part 1 of the report here.

Key Points:

  • PRRS virus can be detected in the environment of the farrowing house (surfaces and air) and on the udder skin of lactating sows. However, PRRSV detection in the environment decreases as time after an outbreak increases.
  • PRRSV was not detected in the environment after 4 months of an outbreak
  • Role of environmental PRRSV in the transmission of the disease is still unknown.

In this study, udder and surface wipes as well as particle deposition wipes were collected both at processing and at weaning, starting 2 weeks after the PRRSV outbreak.

PRRS sampling udder wipes surface wipes particle deposition

Results showed that PRRSV was detected at processing up to 14 weeks after the outbreak in surfaces and udder skin of lactating sows. At weaning, PRRSV was detected up to 17 weeks post-outbreak using udder skin wipes. The number of positive samples decreased over time and the Ct values of the positive samples increased over time indicating a decrease in infection load overtime. Detection of airborne particle deposition positive samples followed a similar pattern to those of the crate surfaces and udder wipes. Virus could be isolated and sequenced from all sample types.

Udder skin and environment may play a role in the transmission and maintenance of PRRSV in piglets in breeding herds; however further research is needed to validate this observation.

 

Swine Global Surveillance Project Issues First Reports

cahfs_primary_graphicThe University of Minnesota Swine Group and the Center for Animal Health and Food Safety (CAHFS) have partnered with the Swine Health Information Center (SHIC) to develop and implement a system for near real time global surveillance of swine diseases. The output of the system is the identification of hazards that are subsequently scored using a step-wise procedure of screening, to identify increments in hazards that, potentially, may represent a risk for the US.

The first version of the system is now live, with the first three reports available, including data from November 5, 2017 to January 14, 2018.

Beginning in early March the tool will be available for spontaneous reporting by stakeholders, such as producers and practitioners both overseas and in the United States. During the first year of the project, the system will be developed and beta-tested for USDA-classified tier 1 reportable foreign animal swine diseases (ASF, CSF, FMD), but in the future more diseases will be tracked.

“As we have learned in recent years, we need to pay attention to external health threats as part of our overall risk management. Keeping tabs on global trends is a prudent investment,” said Dr. Jerry Torrison, Director of the University of Minnesota Veterinary Diagnostic Laboratory.

From the most recent report, December 18, 2017 – January 14, 2018:

The current concern continues to focus on African swine fever in Poland and surrounding countries. Infected wild boars continue to be identified in the vicinity surrounding Warsaw, and the possibility of spread of the disease to the pig intensive area of eastern Poland continues to be a concern. Countries in the region are using a combination of increased hunting of wild boar along with boar proof fencing along borders to attempt to control the spread of the disease.

Visit z.umn.edu/SwineGlobalSurveillance to access the reports, and coming soon, to use the tool to provide spontaneous reporting.

AGREETT funding creates a renaissance in agricultural programs at the University of Minnesota

In 2015, the Minnesota State Legislature created the Agricultural Research, Education, Extension, and Technology Transfer program (AGREETT). Funding was established by the Department of Agriculture to support scientists and educators, increase the next generation of agricultural innovation and enhance Minnesota’s agricultural economy.

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In 2017, several AGREETT experts  were hired:

  • Matthew Aliota, Assistant Professor, Department of Veterinary and Biomedical Sciences – Aliota is expected to arrive in February 2018 and will collaborate on interdisciplinary research connecting insect-borne disease to animal health.
  • Erin Cortus, Assistant Professor and Extension Engineer, Bioproducts and Biosystems Engineering – Cortus’s research interests are the measurement and estimation of farm-level gas emissions and the related impacts on animals, workers and surrounding community.
  • Diane DeWitte, Extension Educator – Swine. DeWitte provides quality assurance certifications and biosecurity education to swine producers and youth exhibitors, collaborates on swine barn environmental research, and assists with research conducted at the University’s swine farms at Waseca and Morris.
  • Andres Gomez, Assistant Professor, Department of Animal Science – Gomez is engaged in studying the factors that shape the composition and function of the microbiome associated to animals and humans.
  • Jared Goplen, Extension Educator – Crops. Goplen focuses primarily in the areas of forage and small grain production and is based at the Morris Regional Extension Office.
  • Joleen Hadrich, Associate Professor and Extension Specialist, Department of Applied Economics – Hadrich’s research focuses on agricultural finance and production economics with an emphasis on farm-level profitability.
  • Annalisa Hultberg, Extension Educator, Food Safety – Hultberg’s focus is on-farm food safety education, outreach and research related to Good Agricultural Practices (GAPs), with fruit and vegetable producers.
  • Yuxin Miao, Associate Professor, Department of Soil, Water and Climate – Miao’s research focuses on precision nitrogen management, especially using proximal, UAV-and satellite-based remote sensing technologies to improve crop nitrogen management in different scales of farming systems, and developing integrated precision crop management systems for high crop yield and resource use efficiencies and protection of the environment.
  • Noelle Noyes, Assistant Professor and Extension Educator, Department of Veterinary, Population Medicine – Noyes arrives in May 2018 and will develop practical and effective models for improving preharvest food safety from production through processing by strengthening partnerships between industry, government and the University.
  • Kim VanderWaal, Assistant Professor and Extension Educator, Department of Veterinary Population Medicine – VanderWaal uses large data sets to better understand antibiotic resistance, food safety and pathogen movements within large agricultural production systems.
  • Megan Webb, Assistant Professor and Extension Educator, Department of Animal Science – Webb will develop collaborative research and Extension programs focused on sustaining productivity growth in the beef industry and engaging with producers and industry to grow this vital part of Minnesota’s economy.
  • Melissa Wilson, Assistant Professor and Extension Specialist, Department of Soil, Water, and Climate – Wilson’s research and Extension programs are in manure management and water quality.

In addition to the personnel described above, AGREETT invested $4.2 million in upgrades and improvements in infrastructure to support our research.

  • University of Minnesota Soil Testing Lab
  • Image Technology for Rapid Detection of Crop Pests and Diseases
  • Forest Research Capacity – Cloquet, Minn.
  • Animal Health and Food Safety Analysis Equipment: “Food Centric Corridor
  • Rapid Agricultural Response Project
  • Highly Pathogenic Avian Influenza Research

Read more

Science Page: Investigating the role of the environment and the lactating sow in PRRSV infections during an outbreak (Part 1)

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week, Dr. Carles Vilalta and Dr. Juan Sanhueza in collaboration with Dr. Montse Torremorell discuss the sensitivity and specificity of sampling the farrowing environment and lactating sows at processing to detect PRRSV in an infected farm.

Key Points:

  • Lactating sows and the farrowing environment can be sources of PRRS virus
  • Sampling the farrowing environment and the udder skin of lactating sows can be used to monitor for PRRSV although the sensitivity is lower than that of serum samples.
  • The farrowing environment and the lactating sow may serve as a source of infection for PRRSV.

Sampling started 2 weeks after a PRRSV outbreak was reported in a sow farm. Sampling was conducted from 10 litters every 3 weeks for a total of 24 weeks. Samples were collected at processing (~ 3 days of age) and included: surface wipes of farrowing crates, surface wipes of the udder skin of lactating sows, blood samples from all piglets within the selected litters.

PRRS sampling in the environment and on the sows.gif
Scatter plot of the individual RT-PCR Ct values in serum (all piglets) compared with those from surfaces (A) and udder skin (B).

PRRSV was detected in the farrowing crate environment and on the skin of the lactating sow at processing. The surface and udder skin wipes were less sensitive at detecting PRRSV than serum PCR at processing. However, in this study all pigs in the litter were bled which is not the standard practice in the field.

The results show that the environment and the lactating sow may serve as a source of
infection for PRRSV, indicating a need to further understand their roles to establish herd level stability.