Evaluation of viral RNA extraction methods to detect PRRSV and IAV from used commercial air filters

Today, we are happy to share a new publication! Dr. Nirmala and colleagues from the University of Minnesota report on a viral RNA extraction method that can be used to detect both the porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) from used farm air filters. This work is published in the Journal of Aerosol Science. Take a look at it!

Highlights

  • Extracting RNA using TRIzol reagent from filter material ground with liquid nitrogen can be used to detect PRRSV and IAV from air filters.
  • IAV was detected in more filters than PRRSV, though PRRSV could be detected in used filters from farms with PRRSV negative status at the time of filter removal.
  • The obtained results shows likely evidence of between farm aerosol spread and the methods derived from this study open up avenues to further investigate regional airborne transmission and risk of virus introduction into farms.
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