pooling – Swine in Minnesota

The impact of pooling on the observed microbiome profile of preweaned piglet feces

Today, we are sharing a recent publication from the Noyes lab investigating a very popular sampling technique, pooling and the impact it has on resulting microbiome profiles. The article is available in open access on Microbiology Ecology’s website.

Alternative Samples for Porcine Reproductive and Respiratory Syndrome Surveillance in an Endemic PRRSV-1-Infected Breeding Herd: A Descriptive Study

This is our Friday rubric: every week a new Science Page from the Bob Morrison’s Swine Health Monitoring Project. The previous editions of the science page are available on our website.

This week Arnaud Lebret, Valérie Normand, Pauline Berton, Théo Nicolazo, Charlotte Teixeira Costa, Céline Chevance, Mathieu Brissonnier and Gwenaël Boulbria share a descriptive study comparing alternative sample types for PRRS type 1 surveillance.

Pooling udder skin wipes to detect influenza in preweaning pigs

This is a recent publication from the Torremorell’s lab refining the use of udder wipes to detect influenza in preweaning pigs. It is available in the Journal of Veterinary Diagnostic Investigation.

Key points

Pooling of three, five, and ten udder wipes was evaluated.
Sensitivity decreased if the Ct count was above 31.5.
Pooling more than three udder wipes can affect the ability to detect influenza virus.

Effect of litter aggregation and pooling on detection of PRRSv in piglet processing fluids

The Science Page this week was written by the MSHMP team and covers pooling of processing fluids. A more detailed report can be found on the blog. Currently, a study to evaluate the number of negative processing fluids to declare a farm stable is ongoing. If you are interested in participating in this study, contact Dr. Juan Sanhueza (jsanhuez-at-umn-dot-edu).