In this concise and straight-forward review for The Veterinary journal, Dr. Garcia-Morante in collaboration with the MycoLab at the UMN shares the latest knowledge around Mycoplasma hyopneumoniae infection and what the best practices for diagnosis are. The full article is available on the journal’s website.
This review updates M. hyopneumoniae infection diagnosis and dynamics.
Understanding M. hyopneumoniae infection dynamics is critical for diagnosis.
Final diagnosis is based on clinical observations aligned with laboratory results.
Laboratory tests are useful for detection of asymptomatic carriers.
Actinobacillus pleuropneumoniae can significantly contribute to increase the costs of the growing period by increasing mortality and antimicrobial treatments.
All-in all-out of the affected sites accompanied with standard cleaning and disinfection procedures may suffice to ensure elimination of the bacteria.
A series of outbreaks with a sudden increase in mortality in growing pig herds located in Northwest Iowa were reported beginning in late October and early November.
Five wean-to-finish farms belonging to three different production companies were affected by a sudden onset (within 12-36 hours) of lethargy, respiratory distress and septicemia across hundreds of pigs. Clinical signs quickly spread through the sites and mortality rapidly increased with pigs having foamy bloody nasal discharge. Post-mortem examination revealed acute pleuritis and severe necrotizing bronchopneumonia. In all cases, Actinobacillus pleuropneumoniae (APP) was cultured from multiple sections of fresh lung. The APP isolate from each case was submitted to the University of Montreal for serotyping and it was confirmed to be serotype 8.
Each veterinarian intervened by rapidly mass injecting the growing herd with antibiotics suggested from the antibiotic susceptibility test together with either in-feed or water medication. Mortality rates for each site are shown on the figure below.
The estimated cost of APP for each of these outbreaks was $30-$35/pig, considering treatment costs and a $2/pig cost for each 1% mortality.
Each site was completely emptied of pigs, washed and disinfected following a standard procedure. Sites were reloaded with new groups of pigs that have remained free of clinical signs associated with APP.
In lieu of the Science Page today, we are bringing you our most popular articles on the blog this past year: a publication by Dr. Maria Pieters, head of the MycoLab called Sample and diagnostic types for early detection of Mycoplasma hyopneumoniae.
Mycoplasma hyopneumoniae is the causative agent enzootic pneumonia, an economically significant disease in pigs. In this study published by Drs. Pieters and Rovira from the University of Minnesota, pigs experimentally inoculated with M.hyopneumoniae were sampled 0, 2, 5, 9, 14, 21, and 28 post-inoculation.
Different sample types were compared:
Using different diagnostic tests:
ELISA IgG anti M.hyopneumoniae
ELISA Ig M anti M.hyopneumoniae
ELISA C-reactive protein
Laryngeal swab samples tested by PCR were highly sensitive for detection of Mycoplasma hyopneumoniae in live pigs. Various commercial ELISA kits for detection of Mycoplasma hyopneumoniae antibodies showed similar sensitivity. Oral fluids showed a low sensitivity for detection of Mycoplasma hyopneumoniae in experimentally infected pigs.
This month in the National Hog Farmer, Drs. Carles Vilalta, Juan Sanhueza, and Montse Torremorell share a project instigated by the late Dr. Bob Morrison regarding the use of processing fluids to make a PRRSV diagnosis.
The improvement of sampling and diagnostics techniques has made sampling on the farm an easier task with the use of pooled serums or oral fluids samples for example.
One of the ways to get cheaper, more sensitive and quicker techniques would be to use routine chores, such as piglet processing, since castration and tail docking are part of the regular procedures in sow farms.
The goal of this study was to evaluate the accuracy of the processing fluids (the liquid accumulated at the bottom of the pail when farmers collect tails and testicles during routine procedures) by real-time polymerase chain reaction to assess PRRSV status in a sow herd.
The key points from the studies were:
• Using processing fluids as a diagnostic tool can help us to detect lower PRRS prevalence in the herd.
• Testicles and tails should be collected in a pail as they are potential spreaders of PRRS in the farrowing room.
• We should target young parity sows for PRRSV sampling.