Comparison of individual, group and environmental sampling strategies to conduct influenza surveillance in pigs

In this new scientific publication from Dr. Jorge Garrido, PhD candidate from the Torremorell lab, numerous sampling strategies to monitor influenza were compared. the following individual, litter, and environmental samples were included in the study:

  • Nasal swabs
  • Nasal wipes
  • Oropharyngeal swabs
  • Oral fluids
  • Surface wipes
  • Udder wipes
  • Airborne particle deposition
  • Air
Continue reading “Comparison of individual, group and environmental sampling strategies to conduct influenza surveillance in pigs”

Sample types and diagnostic methods for early detection of Mycoplasma hyopneumoniae

In lieu of the Science Page today, we are bringing you our most popular articles on the blog this past year: a publication by Dr. Maria Pieters, head of the MycoLab called Sample and diagnostic types for early detection of Mycoplasma hyopneumoniae.

Summary:

Mycoplasma hyopneumoniae is the causative agent enzootic pneumonia, an economically significant disease in pigs. In this study published by Drs. Pieters and Rovira from the University of Minnesota, pigs experimentally inoculated with M.hyopneumoniae were sampled 0, 2, 5, 9, 14, 21, and 28 post-inoculation.

Different sample types were compared:

  • Nasal swabs
  • Laryngeal swabs
  • Tracheobronchal lavages
  • Oral fluids
  • Serum samples

Using different diagnostic tests:

  • PCR
  • ELISA IgG anti M.hyopneumoniae
  • ELISA Ig M anti M.hyopneumoniae
  • ELISA C-reactive protein

Laryngeal swab samples tested by PCR were highly sensitive for detection of Mycoplasma hyopneumoniae in live pigs. Various commercial ELISA kits for detection of Mycoplasma hyopneumoniae antibodies showed similar sensitivity. Oral fluids showed a low sensitivity for detection of Mycoplasma hyopneumoniae in experimentally infected pigs.

Link to the full-article