Infection dynamics and genetic variability of Mycoplasma hyopneumoniae in self-replacement gilts

This is a new research paper from the MycoLab under Dr. Maria Pieters’ supervision. In this study, the group looked at the infection dynamics and genetic variability of Mycoplasma hyopneumoniae in self-replacement gilts, in 3 positive herds. Serum samples were taken from the gilts at 150 days of age onward and laryngeal swabs were collected from the gilts and their progeny.

Highlights of this project

  • Genetic variability of M. hyopneumoniae was evaluated using MLVA typing.
  • The highest M. hyopneumoniae prevalence in gilts was detected at 150┬ádays of age.
  • Detection patterns for M.hyopneumoniae were different among farms.
  • Genetic variability was identified within and among farms.

 

Pieters 2017 infection dynamics Mhyop

Abstract:

The aim of this study was to assess the longitudinal pattern of M. hyopneumoniae detection in self-replacement gilts at various farms and to characterize the genetic diversity among samples. A total of 298 gilts from three M. hyopneumoniae positive farms were selected at 150 days of age (doa). Gilts were tested for M. hyopneumoniae antibodies by ELISA, once in serum at 150 doa and for M. hyopneumoniae detection in laryngeal swabs by real time PCR two or three times. Also, 425 piglets were tested for M. hyopneumoniae detection in laryngeal swabs. A total of 103 samples were characterized by Multiple Locus Variable-number tandem repeats Analysis. Multiple comparison tests were performed and adjusted using Bonferroni correction to compare prevalence of positive gilts by ELISA and real time PCR. Moderate to high prevalence of M. hyopneumoniae in gilts was detected at 150 doa, which decreased over time, and different detection patterns were observed among farms. Dam-to-piglet transmission of M. hyopneumoniae was not detected. The characterization of M. hyopneumoniae showed 17 different variants in all farms, with two identical variants detected in two of the farms. ELISA testing showed high prevalence of seropositive gilts at 150 doa in all farms. Results of this study showed that circulation of M. hyopneumoniae in self-replacement gilts varied among farms, even under similar production and management conditions. In addition, the molecular variability of M. hyopneumoniae detected within farms suggests that in cases of minimal replacement gilt introduction bacterial diversity maybe farm specific.

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